Vanoosthuyze K, Daeseleire E, Van Overbeke A, Van Peteghem C, Ermens A
Laboratory of Food Analysis, University of Ghent, Belgium.
Analyst. 1994 Dec;119(12):2655-8. doi: 10.1039/an9941902655.
Although the illegal use of orally administered compounds in cattle fattening has gained popularity, injection sites are still frequently found during control experiments on the carcasses in the slaughterhouses. The high concentrations of hormones in injection sites enable screening for the presence of 39 different hormones by a simple extraction followed by a fast and simple high-performance thin-layer chromatography analysis. Analysis of injection-site tissue is particularly successful for determining the hormones that are illegally injected. This data can not be obtained by analysis of other biological matrices like faeces, kidney fat or urine, owing to metabolization and selective excretion and/or deposition of these compounds. Since 1989, over 2000 injection sites have been analysed in our laboratory, which yielded a good survey of the hormones that were illegally injected. Over this period, the natural hormones estradiol and testosterone (mostly present as their esters) have obviously been used extensively. It is clear that since 1990 clostebol acetate has remained the most abused exogenous hormone. Additionally, some distinct trends were noticed, e.g., a tendency towards a highly decreased use of nandrolone, an increased use of progesterone and an increased occurrence of certain androgens like stanozolol and fluoxymesterone.
尽管非法使用口服化合物育肥牛的情况日益普遍，但在屠宰场对屠体进行控制检测时，仍经常发现注射部位。注射部位的激素浓度很高，通过简单提取，然后进行快速简便的高效薄层色谱分析，能够筛查出39种不同的激素。对注射部位组织进行分析，对于确定非法注射的激素特别有效。由于这些化合物的代谢、选择性排泄和/或沉积，通过分析粪便、肾脂肪或尿液等其他生物基质无法获得此数据。自1989年以来，我们实验室已分析了2000多个注射部位，从而对非法注射的激素有了很好的了解。在此期间，天然激素雌二醇和睾酮（大多以其酯的形式存在）显然被广泛使用。很明显，自1990年以来，醋酸氯睾酮一直是滥用最严重的外源激素。此外，还注意到一些明显的趋势，例如，诺龙的使用量大幅下降、孕酮的使用量增加，以及司坦唑醇和氟甲睾酮等某些雄激素的出现频率增加。
