采用气相色谱-氮磷检测器和气相色谱-质谱联用技术测定马尿中的丁二腈。

Determination of succinonitrile in horse urine by gas chromatography-nitrogen-phosphorus detector and gas chromatography-mass spectrometry.

作者信息

Pedroso R C, Salvadori M C, Andraus M H, Lopez N M

机构信息

Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil.

出版信息

Analyst. 1994 Dec;119(12):2697-9. doi: 10.1039/an9941902697.

DOI:10.1039/an9941902697

PMID:7879879

Abstract

A chromatographic method was developed to detect and confirm the presence of succinonitrile (SDN) in horse urine samples, for antidoping control. The urine samples (5 ml) were extracted with diethyl ether and screened by gas chromatography-nitrogen-phosphorus detector and the confirmation of the drug's presence was accomplished by using gas chromatography-mass selective detection. The recovery of extraction was 78 and 81% for 1.0 and 2.0 micrograms ml-1 (relative standard deviation, < 10%), respectively. Urine samples collected after the administration of Energisan were positive for SDN (1-30 h) in all samples analysed.

摘要

开发了一种色谱方法，用于在反兴奋剂控制中检测和确认马尿样中丁二腈（SDN）的存在。取5毫升尿样用乙醚萃取，通过气相色谱-氮磷检测器进行筛查，并使用气相色谱-质量选择性检测来确认药物的存在。对于1.0和2.0微克/毫升的浓度，萃取回收率分别为78%和81%（相对标准偏差<10%）。在分析的所有样本中，服用Energisan后采集的尿样在给药后1至30小时内SDN检测呈阳性。