Experimental infection of the bovine udder with the induced L phase of Streptococcus agalactiae.

Two out of 30 strains of Streptococcus agalactiae produced stable L phase variants when passaged on hypertonic agar containing penicillin. Streptococcal L phase variants were inoculated into six quarters of cows. The cows were observed for three months but the L phase was not reisolated and no reversion took place, the cows remaining free from streptococcal infection.