Ishiyama A, Lopez I, Wackym P A
Temporal Bone and Molecular Biology Laboratories, Goodhill Ear Center, UCLA School of Medicine 90024-1794.
Cell Biol Int. 1994 Oct;18(10):979-84. doi: 10.1006/cbir.1994.1019.
Acetylcholine (ACh) is believed to play a major role in the efferent vestibular system in several animal models, however no information regarding the role of ACh in the human efferent vestibular system has been published. Post-embedding immunohistochemistry in a hydrophilic resin was used to investigate the choline acetyltransferase immunoreactivity (ChATi) and acetylcholinesterase (AChE) histochemistry in human vestibular end-organs. ChATi and AChE activity was found in numerous bouton-type terminals at the basal area of the vestibular hair cells. These terminals were found to contact type II vestibular hair cells and the afferent chalices surrounding type I hair cells. This study provides the first evidence that the human efferent vestibular axons and terminals are cholinergic.
在几种动物模型中，乙酰胆碱（ACh）被认为在前庭传出系统中起主要作用，然而，尚未有关于ACh在人类前庭传出系统中作用的相关信息发表。采用亲水性树脂包埋后免疫组织化学方法，研究人类前庭终器中的胆碱乙酰转移酶免疫反应性（ChATi）和乙酰胆碱酯酶（AChE）组织化学。在前庭毛细胞基部区域的许多纽扣型终末中发现了ChATi和AChE活性。这些终末被发现与II型前庭毛细胞以及围绕I型毛细胞的传入杯状小体相接触。本研究首次提供了人类前庭传出轴突和终末是胆碱能的证据。
