Ishiyama A, Lopez I, Wackym P A
Goodhill Ear Center, Section of Otology, 90024-1794.
Otolaryngol Head Neck Surg. 1994 Oct;111(4):385-95. doi: 10.1177/019459989411100402.
We examined the ultrastructural distribution of calcitonin gene-related peptide immunoreactivity in the peripheral vestibular system of the chinchilla to study the innervation patterns of this efferent neuropeptide. Immunoelectron microscopic localization of calcitonin gene-related peptide immunoreactive terminals in the maculae and cristae revealed an extensive innervation pattern on the afferent vestibular pathway. Calcitonin gene-related peptide immuno-reactive terminals made synaptic contacts with the unmyelinated portions of the primary afferent vestibular dendrites innervating both type I and type II hair cells. Abundant synaptic contact between calcitonin gene-related peptide immunoreactive terminals and the chalices surrounding type I hair cells was observed. Direct contact between calcitonin gene-related peptide immunoreactive terminals and type II hair cells was observed. In addition, vesiculated efferent terminals without calcitonin gene-related peptide immunoreactivity were seen synapsing on the chalices of type II hair cells and on the surrounding type I hair cells. The primary afferent somata in the vestibular ganglion of Scarpa did not contain calcitonin gene-related peptide immunoreactivity. Unmyelinated calcitonin gene-related peptide immunoreactive axons passed among the primary afferent fibers in Scarpa's ganglion, and these fibers continued through the subepithelial regions of the vestibular end-organs. The calcitonin gene-related peptide immunoreactive axons ramified to produce numerous calcitonin gene-related peptide immunoreactive terminals throughout the neurosensory epithelium of the maculae and cristae. These data suggest that calcitonin gene-related peptide-mediated modulation of the afferent vestibular system is functionally important.
我们研究了降钙素基因相关肽免疫反应性在毛丝鼠外周前庭系统中的超微结构分布，以探讨这种传出神经肽的神经支配模式。降钙素基因相关肽免疫反应性终末在黄斑和嵴中的免疫电子显微镜定位显示，在前庭传入通路上存在广泛的神经支配模式。降钙素基因相关肽免疫反应性终末与支配I型和II型毛细胞的前庭初级传入树突的无髓部分形成突触联系。观察到降钙素基因相关肽免疫反应性终末与围绕I型毛细胞的杯状细胞之间有丰富的突触联系。观察到降钙素基因相关肽免疫反应性终末与II型毛细胞直接接触。此外，还可见到无降钙素基因相关肽免疫反应性的含囊泡传出终末与II型毛细胞的杯状细胞以及周围的I型毛细胞形成突触。斯卡帕前庭神经节中的初级传入神经元胞体不含降钙素基因相关肽免疫反应性。无髓的降钙素基因相关肽免疫反应性轴突在斯卡帕神经节的初级传入纤维之间穿行，这些纤维继续穿过前庭终器的上皮下区域。降钙素基因相关肽免疫反应性轴突分支，在黄斑和嵴的整个神经感觉上皮中产生大量降钙素基因相关肽免疫反应性终末。这些数据表明，降钙素基因相关肽介导的前庭传入系统调节在功能上很重要。
