Yull F E, Wallace R M, Clark A J
Roslin Institute, Edinburgh Research Station, Roslin, Midlothian EH25 9PS, Scotland, UK.
Transgenic Res. 1995 Jan;4(1):70-4. doi: 10.1007/BF01976504.
The tissue-specific and developmental pattern of expression controlled by the proximal promoter (position-348 to +15) derived from the human alpha-1-antitrypsin (h alpha 1AT) gene was studied in transgenic mice. The short promoter segment was linked to the chloramphenicol acetyltransferase (CAT) reporter gene. The transgene showed highly specific expression in the liver and the correct developmental pattern of regulation. Interestingly, this short promoter targets expression to the liver with a greater specificity than that reported for larger alpha 1AT promoter fragments.
在转基因小鼠中研究了源自人α-1-抗胰蛋白酶（hα1AT）基因的近端启动子（位置-348至+15）所控制的组织特异性和发育性表达模式。该短启动子片段与氯霉素乙酰转移酶（CAT）报告基因相连。转基因在肝脏中表现出高度特异性表达以及正确的发育调控模式。有趣的是，与报道的较大α1AT启动子片段相比，这个短启动子将表达靶向肝脏的特异性更高。
