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人类α1-抗胰蛋白酶基因在巨噬细胞和肝细胞中由两个不同的启动子转录。

The human alpha 1-antitrypsin gene is transcribed from two different promoters in macrophages and hepatocytes.

作者信息

Perlino E, Cortese R, Ciliberto G

机构信息

European Molecular Biology Laboratory, Heidelberg, FRG.

出版信息

EMBO J. 1987 Sep;6(9):2767-71. doi: 10.1002/j.1460-2075.1987.tb02571.x.

DOI:10.1002/j.1460-2075.1987.tb02571.x
PMID:3500042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553701/
Abstract

In order to investigate the mechanism of expression of the human alpha 1-antitrypsin (alpha 1-AT) gene in macrophages, we have characterized the alpha 1-AT transcriptional units in these cells and discovered that there is a macrophage-specific promoter located approximately 2000 bp upstream of the hepatocyte-specific promoter. Transcription from the two alpha 1-AT promoters is mutually exclusive: the macrophage promoter is silent in hepatocytes and the hepatocyte promoter is silent in macrophages. In addition, in macrophages two distinct mRNAs are generated transcript by alternative splicing. These results suggest that alpha 1-AT gene transcription responds to two different cell-specific regulatory mechanisms.

摘要

为了研究人类α1 -抗胰蛋白酶（α1 -AT）基因在巨噬细胞中的表达机制，我们对这些细胞中的α1 -AT转录单位进行了特征分析，发现存在一个巨噬细胞特异性启动子，其位于肝细胞特异性启动子上游约2000 bp处。来自两个α1 -AT启动子的转录是相互排斥的：巨噬细胞启动子在肝细胞中沉默，而肝细胞启动子在巨噬细胞中沉默。此外，在巨噬细胞中，通过可变剪接产生两种不同的mRNA转录本。这些结果表明，α1 -AT基因转录对两种不同的细胞特异性调节机制产生反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9189/553701/ef02101cb192/emboj00249-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9189/553701/bfb4274119e1/emboj00249-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9189/553701/ef02101cb192/emboj00249-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9189/553701/bfb4274119e1/emboj00249-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9189/553701/ef02101cb192/emboj00249-0256-a.jpg

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2
Structure and variation of human alpha 1-antitrypsin.人类α1-抗胰蛋白酶的结构与变异
Nature. 1982 Jul 22;298(5872):329-34. doi: 10.1038/298329a0.
3
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Int J Mol Sci. 2022 Feb 23;23(5):2441. doi: 10.3390/ijms23052441.
4
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