Miranda C L, Schoor W P, Zhao X, Henderson M C, Reed R L, Buhler D R
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1994 Sep;109(1):27-35.
Immunoblots (Western blot) of mullet liver microsomes revealed the presence of multiple forms of P-450 that appear to be structurally related to rainbow trout CYP1A1 and CYP2K1 and to P-450 LMC1 and LMC4, but not to LMC5. 3-Methylcholanthrene but not beta-naphthoflavone induced a major 58 kDa liver protein and a minor 56 kDa protein in mullet that both cross-reacted with anti-trout CYP1A1 IgG. The levels of immunodetectable P-450s and the activities of microsomal lauric acid hydroxylase, DMBA hydroxylase and progesterone 6 beta-hydroxylase were several times lower in mullet liver than in rainbow trout liver; however, progesterone 16 alpha-hydroxylase and progesterone 20 alpha-hydroxylase activities were 4-fold and 6-fold higher, respectively, in mullet than in trout liver.
鲻鱼肝脏微粒体的免疫印迹（蛋白质印迹法）显示存在多种形式的细胞色素P-450，它们在结构上似乎与虹鳟鱼的CYP1A1和CYP2K1以及P-450 LMC1和LMC4相关，但与LMC5无关。3-甲基胆蒽而非β-萘黄酮诱导鲻鱼肝脏中一种主要的58 kDa蛋白质和一种次要的56 kDa蛋白质，这两种蛋白质均与抗鳟鱼CYP1A1免疫球蛋白发生交叉反应。鲻鱼肝脏中可免疫检测到的细胞色素P-450水平以及微粒体月桂酸羟化酶、二甲基苯并蒽羟化酶和孕酮6β-羟化酶的活性比虹鳟鱼肝脏低几倍；然而，鲻鱼肝脏中孕酮16α-羟化酶和孕酮20α-羟化酶的活性分别比鳟鱼肝脏高4倍和6倍。
