Wang Q P, Guan J L, Nakai Y
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Brain Res. 1994 Nov 28;665(1):39-46. doi: 10.1016/0006-8993(94)91149-5.
The pre-embedding double immunoreaction method was used to study synaptic relations of enkephalinergic and GABAergic neuronal elements in the ventrolateral part of the periaqueductal gray of the Wistar albino rat. The enkephalin-like neuronal elements were immunoreacted by the silver-gold intensified peroxidase-antiperoxidase method and the GABA-like immunoreactive neurons were immunoreacted by the unintensified peroxidase-antiperoxidase method. GABA-like immunoreactive neuronal somata were post-synaptic to both the enkephalin-like immunoreactive and the non-immunoreactive axon terminals. Enkephalin-like immunoreactive axon terminals were found to make synapses with GABA-like immunoreactive and non-immunoreactive dendrites. The synapses between the two kinds of chemically characterized neurons appeared to be both asymmetrical and symmetrical. Possible functional activity related to pain modulation, and synaptic relations between the enkephalinergic and GABAergic neurons in the periaqueductal gray and the dorsal raphe nucleus, are discussed.
采用包埋前双重免疫反应法研究Wistar白化大鼠中脑导水管周围灰质腹外侧部脑啡肽能和γ-氨基丁酸能神经元成分的突触关系。脑啡肽样神经元成分采用银增强过氧化物酶-抗过氧化物酶法进行免疫反应，γ-氨基丁酸样免疫反应神经元采用未增强的过氧化物酶-抗过氧化物酶法进行免疫反应。γ-氨基丁酸样免疫反应神经元胞体是脑啡肽样免疫反应和非免疫反应轴突终末的突触后成分。发现脑啡肽样免疫反应轴突终末与γ-氨基丁酸样免疫反应和非免疫反应树突形成突触。两种化学特性不同的神经元之间的突触似乎既有不对称的，也有对称的。讨论了与疼痛调制相关的可能功能活动，以及中脑导水管周围灰质和背侧中缝核中脑啡肽能和γ-氨基丁酸能神经元之间的突触关系。
