Yazawa S, Nishihara S, Iwasaki H, Asao T, Nagamachi Y, Matta K L, Narimatsu H
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
Cancer Res. 1995 Apr 1;55(7):1473-8.
It has been observed that the frequency of individuals with Lewis-negative erythrocytes is significantly higher in cancer patients than in healthy controls. In this study, 20 of the 66 (30.3%) patients with various cancers were typed as Lewis negative from their erythrocytes, while the same frequency in healthy controls was 11.1%. These 20 patients were divided into three groups based on the presence of Lewis blood group antigens and alpha 1-->4-fucosyltransferase in their salivas: group I, 6 patients who had both Lewis antigens and alpha 1-->4-fucosyltransferase activity; group II, 8 patients who had no Lewis antigens but possessed alpha 1-->4-fucosyltransferase activity; group III, 6 patients who had neither Lewis antigens nor alpha 1-->4-fucosyltransferase activity. The genotyping of Le genes by the PCR-RFLP methods, which have been developed and established by us recently, demonstrated that all 14 patients from groups I and II possess Le gene homozygously (Le/Le) or heterozygously (Le/le), whereas all 6 patients from group III were le/le homozygotes. Only the 6 patients from group III were identified as the genuine Lewis-negative individuals. The immunohistochemical staining of the colorectal tumors also showed that the Lewis antigens could be detected on the tumors from groups I and II but not from group III.
据观察，癌症患者中红细胞Lewis阴性个体的频率显著高于健康对照组。在本研究中，66例各类癌症患者中有20例（30.3%）红细胞血型为Lewis阴性，而健康对照组的这一频率为11.1%。根据唾液中Lewis血型抗原和α1→4-岩藻糖基转移酶的存在情况，将这20例患者分为三组：第一组，6例同时具有Lewis抗原和α1→4-岩藻糖基转移酶活性；第二组，8例无Lewis抗原但具有α1→4-岩藻糖基转移酶活性；第三组，6例既无Lewis抗原也无α1→4-岩藻糖基转移酶活性。我们最近开发并建立的PCR-RFLP方法对Le基因进行基因分型，结果显示，第一组和第二组的所有14例患者均为Le基因纯合子（Le/Le）或杂合子（Le/le），而第三组的所有6例患者均为le/le纯合子。只有第三组的6例患者被确定为真正的Lewis阴性个体。结直肠癌的免疫组化染色也显示，第一组和第二组的肿瘤上可检测到Lewis抗原，而第三组的肿瘤上未检测到。
