Ebisawa T, Sasaki Y, Deguchi T
Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Eur J Biochem. 1995 Feb 15;228(1):129-37. doi: 10.1111/j.1432-1033.1995.tb20240.x.
A cDNA library was constructed from the pineal gland of rats injected with isoproterenol and screened with 32P-labeled cDNAs encoding arylamine N-acetyltransferases from rabbit and human liver. Two types of cDNAs for arylamine N-acetyltransferases (A-type and B-type) were isolated. Expression of the cDNAs in Chinese hamster ovary cells indicated that A-type N-acetyltransferase acetylates both arylamines and arylalkylamines, while the B-type enzyme acetylates only arylamines. Therefore, neither the A-type nor the B-type of enzyme seems to be the arylalkylamine N-acetyltransferase involved in melatonin synthesis in the pineal gland. Nucleotide sequence analysis revealed that both A-type and B-type cDNAs code for 290 amino acids, and that they showed 82.8% similarity in the coding region. However, the nucleotide sequence in the 5' non-coding region was identical in the A-type and B-type cDNAs. In addition, the 5' non-coding region contained another possible open reading frame for 79 amino acids. Data base research revealed that the complementary sequence of the 5' non-coding region has high similarity with the coding regions of cDNAs for high-mobility-group proteins (HMG) 1 and 2, which are thought to regulate mRNA transcription.
用异丙肾上腺素注射大鼠，从其松果体构建了一个cDNA文库，并用来自兔和人肝脏的编码芳胺N - 乙酰基转移酶的32P标记的cDNA进行筛选。分离出两种类型的芳胺N - 乙酰基转移酶cDNA（A 型和B型）。在中国仓鼠卵巢细胞中cDNA的表达表明，A型N - 乙酰基转移酶可使芳胺和芳烷基胺乙酰化，而B型酶仅使芳胺乙酰化。因此，无论是A型还是B型酶似乎都不是松果体中参与褪黑素合成的芳烷基胺N - 乙酰基转移酶。核苷酸序列分析显示，A型和B型cDNA均编码290个氨基酸，并且它们在编码区显示出82.8%的相似性。然而，A型和B型cDNA的5'非编码区的核苷酸序列是相同的。此外，5'非编码区包含另一个可能编码79个氨基酸的开放阅读框。数据库研究表明，5'非编码区的互补序列与高迁移率族蛋白（HMG）1和2的cDNA编码区具有高度相似性，而HMG 1和2被认为可调节mRNA转录。
