Liyanage H, Penfold C, Turner J, Bender C L
Department of Plant Pathology, Oklahoma State University, Stillwater 74078-9947.
Gene. 1995 Feb 3;153(1):17-23. doi: 10.1016/0378-1119(94)00661-b.
Pseudomonas syringae pv. glycinea PG4180 produces the chlorosis-inducing phytotoxin coronatine (COR), which consists of a polyketide component, coronafacic acid (CFA), ligated by an amide bond to coronamic acid (CMA), an ethylcyclopropyl amino-acid derived from isoleucine. We report the nucleotide sequence of a 2.37-kb region containing the coronafacate ligase-encoding gene (cfl) which is required for the amide linkage of CFA and CMA. The transcription start point for cfl was identified, and the Cfl protein was overproduced from the T7lac promoter in Escherichia coli. The deduced amino-acid sequence of Cfl showed homology to a variety of adenylate-forming enzymes which bind and hydrolyze ATP in order to activate their substrates for further ligation.
丁香假单胞菌大豆致病变种PG4180产生诱导萎黄病的植物毒素冠毒素（COR），它由一个聚酮化合物组分冠腐酸（CFA）和通过酰胺键与冠氨酸（CMA）连接而成，CMA是一种源自异亮氨酸的乙基环丙基氨基酸。我们报道了一个2.37 kb区域的核苷酸序列，该区域包含冠腐酸连接酶编码基因（cfl），CFA和CMA的酰胺连接需要该基因。确定了cfl的转录起始点，并且在大肠杆菌中从T7lac启动子过量表达了Cfl蛋白。Cfl推导的氨基酸序列与多种形成腺苷酸的酶具有同源性，这些酶结合并水解ATP以激活其底物进行进一步连接。
