Cloeckaert A, Jacques I, Limet J N, Dubray G
Institut National de la Recherche Agronomique, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
J Med Microbiol. 1995 Mar;42(3):200-8. doi: 10.1099/00222615-42-3-200.
The immunogenicity of several Brucella melitensis cell-wall (CW) fractions was tested in BALB/c mice. These CW fractions were smooth lipopolysaccharide (S-LPS) fraction from smooth (S) B. melitensis strain 16M, sodium dodecyl sulphate-insoluble (SDS-I) CW fraction from B. melitensis strain 16M (S) undigested or digested with pepsin, and SDS-I CW fraction from rough (R) B. melitensis strain H38. The B. melitensis SDS-I CW fraction contained two major outer-membrane proteins (OMPs) of 25-27 kDa and 31-34 kDa, peptidoglycan (PG) and a small quantity (1.5%) of LPS. One month after immunisation, mice were challenged with virulent B. melitensis strain H38 (S) and Brucella spleen counts were recorded on days 28 and 49 after challenge. Before challenge, as measured by ELISA, the highest antibody responses to S-LPS were observed in mice immunised with SDS-I CW fraction from B. melitensis strain 16M (S), whether digested with pepsin or undigested. All immunised mice, except those immunised with the SDS-I CW fraction from the R strain, showed higher IgG1 than IgG2a antibody responses to S-LPS (IgG1:IgG2a ratio 3.64-7.71). Antibody responses to the 25-27-kDa OMP were very low, with the highest responses in the mice immunised with the SDS-I CW fraction from the R strain. These results indicated that, in BALB/c mice, these CW fractions probably induced Th2-dependent more than Th1-dependent antibody responses.(ABSTRACT TRUNCATED AT 250 WORDS)
在BALB/c小鼠中测试了几种羊布鲁氏菌细胞壁（CW）组分的免疫原性。这些CW组分包括来自光滑型（S）羊布鲁氏菌16M菌株的光滑脂多糖（S-LPS）组分、来自羊布鲁氏菌16M（S）菌株未消化或经胃蛋白酶消化的十二烷基硫酸钠不溶性（SDS-I）CW组分，以及来自粗糙型（R）羊布鲁氏菌H38菌株的SDS-I CW组分。羊布鲁氏菌SDS-I CW组分包含两种主要的外膜蛋白（OMP），分子量分别为25 - 27 kDa和31 - 34 kDa、肽聚糖（PG）以及少量（1.5%）的LPS。免疫后一个月，用强毒株羊布鲁氏菌H38（S）攻击小鼠，并在攻击后第28天和第49天记录脾脏中的布鲁氏菌数量。在攻击前，通过ELISA检测发现，在用来自羊布鲁氏菌16M（S）菌株的SDS-I CW组分免疫的小鼠中，无论是否经胃蛋白酶消化，对S-LPS的抗体反应最高。除了用R菌株的SDS-I CW组分免疫的小鼠外，所有免疫小鼠对S-LPS的IgG1抗体反应均高于IgG2a抗体反应（IgG1:IgG2a比值为3.64 - 7.71）。对25 - 27 kDa OMP的抗体反应非常低，在用R菌株的SDS-I CW组分免疫的小鼠中反应最高。这些结果表明，在BALB/c小鼠中，这些CW组分可能诱导了更多依赖Th2而非依赖Th1的抗体反应。（摘要截短至250字）
