Kvitnitskaya-Ryzhova T, Shinkai T, Ooka H, Ohtsubo K
Laboratory of Morphology and Cytology, Ukrainian Academy of Medical Sciences, Kiev.
Mech Ageing Dev. 1994 Oct 20;76(2-3):65-72. doi: 10.1016/0047-6374(94)91582-2.
Prolactin (PRL) exerts a direct effect on the central nervous system, reaching the PRL-responsive brain regions via cerebro-spinal fluid (CSF). The hormone enters the CSF by a specific receptor-mediated transport mechanism that is localized on the epithelium of the choroid plexus (CP) of brain ventricles. PRL interactions with the CP in aging were examined in young (3-month) and old (27-month) female Wistar rats using immunocytochemistry (immunogold technique). The enhancement of PRL uptake by the CP in animals at both ages was achieved by the modelling of acute hyperprolactinemia. A great age-related difference was found in the intensity of immunocytochemical reaction under activated conditions, the uptake of PRL by CP being significantly higher in young animals than in old. The character of the colloidal gold particle distribution in different components of CP epithelial cells appeared to be the same in both age groups. The weakening of PRL-transporting capacity in the CP of old animals may constitute one aspect of the alteration of neuroendocrine regulation in the CP-CSF system that occurs during aging.
催乳素（PRL）对中枢神经系统有直接作用，它通过脑脊液（CSF）到达对PRL有反应的脑区。该激素通过一种特定的受体介导转运机制进入脑脊液，这种机制定位于脑室脉络丛（CP）的上皮细胞。使用免疫细胞化学（免疫金技术）在年轻（3个月）和年老（27个月）雌性Wistar大鼠中研究了衰老过程中PRL与CP的相互作用。通过模拟急性高催乳素血症，在两个年龄段的动物中均实现了CP对PRL摄取的增强。在激活条件下，免疫细胞化学反应强度存在很大的年龄相关差异，年轻动物中CP对PRL的摄取明显高于年老动物。在两个年龄组中，CP上皮细胞不同成分中胶体金颗粒的分布特征似乎相同。年老动物CP中PRL转运能力的减弱可能是衰老过程中CP - CSF系统神经内分泌调节改变的一个方面。
