肺的重氮复制：一种改进的方法。
Diazo replication of the lung: an improved methodology.
作者信息
Yutani R M, Sherwin R P
出版信息
Am Rev Respir Dis. 1976 Sep;114(3):647-50. doi: 10.1164/arrd.1976.114.3.647.
PMID:788573
Abstract
The methodology of diazo replication has been improved to provide replicas of 60 mum-thick lung sections with high contrast and sharp delineation of airways and air spaces. The newly introduced techniques are critical temperature control, embedment in isopentane, Bismarck brown staining, and parallel light, halogen lamp illumination.
摘要
重氮复制法已得到改进，可提供厚度为60微米的肺切片复制品，气道和肺泡腔对比度高且轮廓清晰。新引入的技术包括临界温度控制、异戊烷包埋、俾斯麦棕染色和平行光卤灯照明。
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