Philippon A, Paul G, Labia R, Nevot P
Ann Microbiol (Paris). 1976 May-Jun;127(4):487-91.
The resistance of Gram negative bacteria to ampicillin and carbenicillin is often associated with the presence of plasmidic transferable beta-lactamase (class III of Richmond and Sykes). Using immunological and physico-chemical tests, two sub-classes have been identified. The sensitivity of Escherichia coli K12 with plasmid P111 (immunotype 1) or plasmid P453 (immunotype 2) determined by means of the minimal inhibitory concentrations, contrasts significantly with a new beta-lactam antibiotic, X-587. Study of the kinetic constants (Km, Vmax) of the beta-lactamases confirms and explains this difference. Thus we have a simple and rapid method for differentiation of these 2 types of enzymes.
革兰氏阴性菌对氨苄青霉素和羧苄青霉素的耐药性通常与质粒介导的可转移β-内酰胺酶（里士满和赛克斯分类中的Ⅲ类）的存在有关。通过免疫学和物理化学检测，已鉴定出两个亚类。用最低抑菌浓度测定法测定，携带质粒P111（免疫型1）或质粒P453（免疫型2）的大肠杆菌K12对一种新型β-内酰胺抗生素X-587的敏感性有显著差异。对β-内酰胺酶动力学常数（Km、Vmax）的研究证实并解释了这种差异。因此，我们有了一种简单快速的方法来区分这两种类型的酶。
