Nishiyama T, Ide T, Himes S R, Ishizaka S, Araki T
Department of Parasitology, Nara Medical University, Japan.
Trans R Soc Trop Med Hyg. 1994 Nov-Dec;88(6):663-5. doi: 10.1016/0035-9203(94)90218-6.
We compared a microcolorimetric enzyme-linked immunosorbent assay (colorimetric ELISA) and a microchemiluminescence enzyme-linked immunosorbent assay (chemiluminescence ELISA) for the detection of specific immunoglobulin G (IgG) in the serum of 9 patients with sparganosis mansoni and 9 healthy controls. The chemiluminescence ELISA was able to measure serum levels of specific IgG over a far wider range than the colorimetric assay, and its detection limit was at least 10-fold lower. An additional 5 sera from sparganosis patients and 5 more from healthy controls, together with sera from 28 patients with other parasitic diseases, were also examined by the chemiluminescence ELISA. All 14 patients with sparaganosis mansoni showed high levels of chemiluminescence (21,302 +/- 18,907 counts per second [cps]). All sera from the 14 healthy controls (1580 +/- 569 cps) and sera from 27 of the 28 patients with other parasites (4 with taeniasis saginata [1767 +/- 501 cps], 11 with diphyllobothriasis latum [1479 +/- 501 cps], 13 with cysticercosis cellulosae [2376 +/- 1437 cps]) showed chemiluminescence levels lower than those of any of the sparganosis mansoni patients. The exception was a patient with cysticercosis (5980 cps), who may have had a dual infection with Cysticercus cellulosae and Sparganum mansoni. Thus, the chemiluminescence ELISA demonstrated high sensitivity and specificity for human sparganosis mansoni.
我们比较了微量比色酶联免疫吸附测定法（比色ELISA）和微量化学发光酶联免疫吸附测定法（化学发光ELISA），用于检测9例曼氏裂头蚴病患者和9例健康对照者血清中的特异性免疫球蛋白G（IgG）。化学发光ELISA能够比色测定法在更宽的范围内测量特异性IgG的血清水平，其检测限至少低10倍。另外5例曼氏裂头蚴病患者的血清和5例健康对照者的血清，以及28例其他寄生虫病患者的血清，也采用化学发光ELISA进行检测。所有14例曼氏裂头蚴病患者均显示出高水平的化学发光（每秒21,302±18,907计数[cps]）。14例健康对照者的所有血清（1580±569 cps）以及28例其他寄生虫病患者中的27例血清（4例牛带绦虫病患者[1767±501 cps]，11例阔节裂头绦虫病患者[1479±501 cps]，13例猪囊尾蚴病患者[2376±1437 cps]）显示的化学发光水平低于任何曼氏裂头蚴病患者。例外的是1例猪囊尾蚴病患者（5980 cps），其可能同时感染了猪囊尾蚴和曼氏裂头蚴。因此，化学发光ELISA对人体曼氏裂头蚴病显示出高敏感性和特异性。
