基于单克隆抗体的竞争酶联免疫吸附测定法对人体裂头蚴病的血清学诊断
Serodiagnosis of human sparganosis by a monoclonal antibody-based competition ELISA.
作者信息
Yeo I S, Yong T S, Im K
机构信息
Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Yonsei Med J. 1994 Mar;35(1):43-8. doi: 10.3349/ymj.1994.35.1.43.
Competition ELISA test using sparganum specific monoclonal antibodies (Mab) was investigated to improve the diagnostic specificity of sparganosis. By cell fusion, one hybridoma clone secreting anti-sparganum specific Mab was selected (Sp-20), which reacted on bands of 32 kDa and 38 kDa. Sp-20 reacted on calcium corpuscles on IFA. By micro-ELISA, 16 of 17 sparganosis cases (95%) were found positive, but 1 of 18 clonorchiasis cases (5%), 4 of 16 cysticercosis cases (25%) and 2 of 16 normal controls (11%) showed false positive reactions. On the other hand, by competition ELISA using a sparganum specific Mab (Sp-20), 16 out of 17 (95%) of sparganosis cases were found positive, but 2 of 18 clonorchiasis cases (10%), 2 of 16 cysticercosis cases (12%), 3 of 16 paragonimiasis cases (18%) and 1 of 16 normal controls (6%) showed false positive reactions.
为提高裂头蚴病的诊断特异性，研究了使用裂头蚴特异性单克隆抗体（Mab）的竞争ELISA试验。通过细胞融合，筛选出一个分泌抗裂头蚴特异性Mab的杂交瘤克隆（Sp-20），其与32 kDa和38 kDa的条带发生反应。Sp-20在间接荧光抗体试验（IFA）中与钙颗粒发生反应。通过微量ELISA，17例裂头蚴病病例中有16例（95%）呈阳性，但18例华支睾吸虫病病例中有1例（5%）、16例囊尾蚴病病例中有4例（25%）以及16例正常对照中有2例（11%）出现假阳性反应。另一方面，使用裂头蚴特异性Mab（Sp-20）进行竞争ELISA时，17例裂头蚴病病例中有16例（95%）呈阳性，但18例华支睾吸虫病病例中有2例（10%）、16例囊尾蚴病病例中有2例（12%）、16例肺吸虫病病例中有3例（18%）以及16例正常对照中有1例（6%）出现假阳性反应。
Zotero 插件