Senoo H, Hata R
Department of Anatomy, School of Medicine, Tokyo Medical and Dental University, Japan.
Kaibogaku Zasshi. 1994 Dec;69(6):719-33.
The roles of extracellular matrix (ECM) components such as collagen, elastin, proteoglycan, and adhesive glycoprotein in the regulation of cell morphology, proliferation, and tissue formation were investigated. On a basement membrane gel, the perisinusoidal stellate cells (lipocytes, fat-storing cells, Ito cells) formed a mesh-like structure, proliferated slowly, and synthesized only a small amount of collagen. On polystyrene or type I collagen-coated culture dishes, the stellate cells spread well and extended cellular processes. The stellate cells proliferated better and synthesized more collagen on type I collagen-coated dishes than on polystyrene dishes. Co-cultures of hepatic parenchymal cells and fibroblasts formed a three-dimensional hepatic cord-like architecture in the medium supplemented with a long-acting vitamin C derivative, L-ascorbic acid 2-phosphate (Asc 2-P). Skin fibroblasts formed a three-dimensional dermis-like structure in the medium supplemented with Asc 2-P. Asc 2-P stimulated collagen synthesis of these cells. The stimulative effects of Asc 2-P on tissue formation were suppressed when collagen synthesis in these cells was inhibited. These data indicate that ECM can regulate cell morphology, proliferation and tissue formation. Regulation of cellular functions in other tissues such as mammary gland, thymus and prostate by ECM was also reviewed, and the molecular mechanisms of the regulation are discussed.
研究了细胞外基质（ECM）成分如胶原蛋白、弹性蛋白、蛋白聚糖和黏附糖蛋白在调节细胞形态、增殖和组织形成中的作用。在基底膜凝胶上，肝血窦周星状细胞（脂肪细胞、贮脂细胞、伊托细胞）形成网状结构，增殖缓慢，仅合成少量胶原蛋白。在聚苯乙烯或I型胶原包被的培养皿上，星状细胞铺展良好并伸出细胞突起。与聚苯乙烯培养皿相比，星状细胞在I型胶原包被的培养皿上增殖更好且合成更多胶原蛋白。在添加长效维生素C衍生物L-抗坏血酸2-磷酸酯（Asc 2-P）的培养基中，肝实质细胞与成纤维细胞的共培养形成了三维肝索样结构。皮肤成纤维细胞在添加Asc 2-P的培养基中形成了三维真皮样结构。Asc 2-P刺激了这些细胞的胶原蛋白合成。当这些细胞中的胶原蛋白合成受到抑制时，Asc 2-P对组织形成的刺激作用也受到抑制。这些数据表明ECM可调节细胞形态、增殖和组织形成。还综述了ECM对乳腺、胸腺和前列腺等其他组织细胞功能的调节作用，并讨论了其调节的分子机制。
