通过快速机械方法制备的酵母线粒体的一些特性。

Some properties of yeast mitochondria prepared by a rapid mechanical procedure.

作者信息

Chambon H, Labbe P

出版信息

Biochimie. 1976;58(7):837-42. doi: 10.1016/s0300-9084(76)80314-8.

Abstract

A method is described for preparing yeast mitochondria rapidly (within one hour) by using the MSK Bronwill Cell homogenizer. Yeast mitochondria obtained by the method exhibit relatively good respiratory controls and ADP/O ratios. The method is convenient for small or large amounts of yeast cells (from 5 to a hundred grams, wet weight) and gave a yield of 3 to 5 mg protein/g wet weight of yeast mitochondria.

摘要

描述了一种使用MSK Bronwill细胞匀浆器快速（在一小时内）制备酵母线粒体的方法。通过该方法获得的酵母线粒体表现出相对良好的呼吸控制和ADP/O比率。该方法适用于少量或大量酵母细胞（湿重5克至100克），酵母线粒体的产量为每克湿重酵母3至5毫克蛋白质。