[丹磺酰二肽基转移核糖核酸与大肠杆菌核糖体的相互作用]

[Interaction of dansyl-dipeptidyl-tRNA with E. coli ribosomes].

作者信息

Vigestane R Ia, Aleksandrova L A, Strel'tsov S A, Viktorova L S, Kukhanova M K

出版信息

Biokhimiia. 1976 Sep;41(9):1641-5.

Abstract

Dansyl-dipeptidyl-tRNA is found to bind with ribosomes in the presence of polyU much more efficiently than AcPhe-tRNA. 30-80% OF introduced danysl-dipeptidyl-tRNA is in a complex with ribosomes and reacts with puromycin in the presence of 5 mM Mg2+. A significant binding of dansyl-dipeptidyl-tRNA both for acceptor and donor ribosome regions is observed in the presence of 10 mMg2+, unlike AcPhe-tRNA, which is capable of the binding only at 20 mM Mg2+. A peptide transfer from dansyl-dipeptidyl-tRNA on puromycin takes place at 10 mM Mg2+ in the absence of template, while AcPhe-tRNA efficiently interacts with puromycin only at 20 mM Mg2+. The data obtained suggest that the increase of hydrophobity of a peptide residue in peptidyl-tRNA increases its binding in peptidyl transferase of ribosomes.

摘要

发现丹磺酰二肽基 - tRNA在多聚U存在的情况下与核糖体结合的效率比乙酰苯丙氨酸 - tRNA高得多。引入的丹磺酰二肽基 - tRNA中有30 - 80%与核糖体形成复合物，并在5 mM Mg2+存在的情况下与嘌呤霉素反应。在10 mM Mg2+存在的情况下，观察到丹磺酰二肽基 - tRNA在受体和供体核糖体区域都有显著结合，这与乙酰苯丙氨酸 - tRNA不同，后者仅在20 mM Mg2+时才能结合。在没有模板的情况下，丹磺酰二肽基 - tRNA上的肽转移到嘌呤霉素发生在10 mM Mg2+时，而乙酰苯丙氨酸 - tRNA仅在20 mM Mg2+时才能有效地与嘌呤霉素相互作用。所获得的数据表明，肽基 - tRNA中肽残基疏水性的增加会增强其在核糖体肽基转移酶中的结合。