一种针对1型人类免疫缺陷病毒gp41的广泛中和性人源单克隆抗体。
A broadly neutralizing human monoclonal antibody against gp41 of human immunodeficiency virus type 1.
作者信息
Purtscher M, Trkola A, Gruber G, Buchacher A, Predl R, Steindl F, Tauer C, Berger R, Barrett N, Jungbauer A
机构信息
Institute of Applied Microbiology, University of Agriculture, Vienna, Austria.
出版信息
AIDS Res Hum Retroviruses. 1994 Dec;10(12):1651-8. doi: 10.1089/aid.1994.10.1651.
We have established a hybridoma clone, designated 2F5, secreting a neutralizing human monoclonal antibody (MAb) specific for gp41 of human immunodeficiency virus type 1 (HIV-1). The epitope of MAb 2F5 was mapped to amino acid sequence Glu-Leu-Asp-Lys-Trp-Ala on the ectodomain of gp41. In this study different in vitro test systems were used to characterize the neutralizing properties of MAb 2F5. In syncytium inhibition assays, fusion inhibition experiments, and neutralization assays on different HIV-susceptible cells (H9, U937, and peripheral blood mononuclear cells) MAb 2F5 showed broad-spectrum neutralizing capacity against HIV-1 laboratory isolates IIIB, MN, RF, and SF2. In addition, primary isolates from AIDS patients were also neutralized.
我们建立了一个名为2F5的杂交瘤克隆，它分泌一种针对1型人类免疫缺陷病毒（HIV-1）gp41的中和性人单克隆抗体（MAb）。单克隆抗体2F5的表位被定位到gp41胞外域的氨基酸序列Glu-Leu-Asp-Lys-Trp-Ala上。在本研究中，使用了不同的体外测试系统来表征单克隆抗体2F5的中和特性。在不同的HIV易感细胞（H9、U937和外周血单个核细胞）的合胞体抑制试验、融合抑制实验和中和试验中，单克隆抗体2F5对HIV-1实验室分离株IIIB、MN、RF和SF2表现出广谱中和能力。此外，来自艾滋病患者的原代分离株也被中和。
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