Tanaka J, Imamura M, Kasai M, Zhu X, Kobayashi S, Imai K, Hashino S, Higa T, Sakurada K, Miyazaki T
Third Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Japan.
Bone Marrow Transplant. 1994 Nov;14(5):695-701.
The response of IFN-gamma, IL-2 and IL-5 mRNA expression to the stimulation of concanavalin A (Con A) in peripheral blood mononuclear cells (PBMC) after bone marrow transplantation (BMT) was analyzed using reverse-transcriptase polymerase chain reaction (RT-PCR) to assess the recovery of T cell function. The subjects were 23 patients undergoing allogeneic BMT, 1 syngeneic BMT, 1 autologous BMT and 2 normal individuals. IFN-gamma mRNA expression increased after Con A stimulation in 6 patients who had limited chronic graft versus host disease (GVHD), 14 patients who did not have chronic GVHD, each one patient receiving syngeneic and autologous BMT and 2 normal individuals. On the other hand, IFN-gamma mRNA expression was not increased by Con A stimulation in 4 patients who had extensive chronic GVHD. Also, the concentration of IFN-gamma in cultured medium in a patient with extensive chronic GVHD was not detectable. A similar low response of IL-2 and IL-5 mRNA expression to Con A was observed in these patients with extensive chronic GVHD. These findings indicate that the cytokine productive capacity of T cell (IFN-gamma and IL-2 could be produced by type 1 T helper (Th1) cells and IL-5 could be produced by type 2 T helper (Th2) cells) was suppressed in patients who had extensive chronic GVHD, while that capacity was almost normal in patients without chronic GVHD and with limited chronic GVHD. Therefore, the analysis of cytokine gene response to Con A stimulation may provide useful information regarding immune reconstitution after BMT.
采用逆转录聚合酶链反应（RT-PCR）分析骨髓移植（BMT）后外周血单个核细胞（PBMC）中干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-5（IL-5）mRNA表达对刀豆蛋白A（Con A）刺激的反应，以评估T细胞功能的恢复情况。研究对象包括23例接受异基因BMT的患者、1例同基因BMT患者、1例自体BMT患者和2名正常个体。在6例慢性移植物抗宿主病（GVHD）局限的患者、14例无慢性GVHD的患者、1例接受同基因和自体BMT的患者以及2名正常个体中，Con A刺激后IFN-γ mRNA表达增加。另一方面，在4例慢性GVHD广泛的患者中，Con A刺激未使IFN-γ mRNA表达增加。此外，1例慢性GVHD广泛的患者培养上清中未检测到IFN-γ浓度。在这些慢性GVHD广泛的患者中，还观察到IL-2和IL-5 mRNA表达对Con A的类似低反应。这些发现表明，在慢性GVHD广泛的患者中，T细胞的细胞因子产生能力（IFN-γ和IL-2可由1型辅助性T细胞（Th1）产生，IL-5可由2型辅助性T细胞（Th2）产生）受到抑制，而在无慢性GVHD和慢性GVHD局限的患者中，该能力几乎正常。因此，分析细胞因子基因对Con A刺激的反应可能为BMT后的免疫重建提供有用信息。
