Vasquez N J, Kane L P, Hedrick S M
Department of Biology, University of California, San Diego, La Jolla 92093-0063.
Immunity. 1994 Apr;1(1):45-56. doi: 10.1016/1074-7613(94)90008-6.
Antigenic stimulation of CD4/CD8 double positive (DP) thymocytes results in programmed cell death, while the identical stimulation of mature T cells results in proliferation and lymphokine secretion. Using thymocytes from transgenic mice expressing pigeon cytochrome c-specific T cell receptors, we previously demonstrated that major histocompatibility complex class II-transfected L cells were capable of presenting peptide antigen and inducing programmed cell death in DP thymocytes, as well as proliferation and lymphokine secretion in mature CD4 single positive (SP) T cells. We therefore were interested in utilizing this system to compare antigen-induced signal transduction events in DP thymocytes and mature SP T cells. In this report, we demonstrate that significant distinctions between thymocytes and mature T cells are seen upon examination of antigen sensitivity and the phosphatidylinositol signaling cascade.
对CD4/CD8双阳性（DP）胸腺细胞的抗原刺激会导致程序性细胞死亡，而对成熟T细胞进行相同刺激则会导致增殖和淋巴因子分泌。利用表达鸽细胞色素c特异性T细胞受体的转基因小鼠的胸腺细胞，我们先前证明，主要组织相容性复合体II类转染的L细胞能够呈递肽抗原，并在DP胸腺细胞中诱导程序性细胞死亡，以及在成熟的CD4单阳性（SP）T细胞中诱导增殖和淋巴因子分泌。因此，我们有兴趣利用该系统比较抗原诱导的DP胸腺细胞和成熟SP T细胞中的信号转导事件。在本报告中，我们证明，在检查抗原敏感性和磷脂酰肌醇信号级联时，可以看到胸腺细胞和成熟T细胞之间存在显著差异。
