Venkataraman L, Francis D A, Wang Z, Liu J, Rothstein T L, Sen R
Rosenstiel Research Center, Brandeis University, Waltham, Massachusetts 02254-9110.
Immunity. 1994 Jun;1(3):189-96. doi: 10.1016/1074-7613(94)90097-3.
Primary B cells are induced to proliferate by cross-linking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor, indistinguishable from the nuclear factor of activated T cells (NF-AT), is induced in B cells in response to anti-immunoglobulin signals or the combined action of phorbol ester and ionomycin, but not in response to either reagent alone. The signals necessary for NF-AT induction in B cells, therefore, closely parallel those required to induce B cell proliferation. Transfection analysis shows that B cell NF-AT is a transcriptional activator. Furthermore, NF-AT induction in splenic cells is suppressed by cyclosporin A, suggesting a mechanism by which immunosuppressive agents act on the B cell compartment. We propose that NF-AT should be considered more generally as a nuclear factor of activated lymphoid cells.
通过交联表面免疫球蛋白或其药理学等效物佛波酯和钙离子载体，可诱导原代B细胞增殖。然而，在活化B细胞中所研究的核反应通常仅用佛波酯即可诱导。我们发现，一种与活化T细胞核因子（NF-AT）无法区分的因子，在B细胞中可因抗免疫球蛋白信号或佛波酯与离子霉素的联合作用而被诱导，但单独使用任何一种试剂均不能诱导。因此，B细胞中诱导NF-AT所需的信号与诱导B细胞增殖所需的信号非常相似。转染分析表明，B细胞NF-AT是一种转录激活因子。此外，环孢素A可抑制脾细胞中NF-AT的诱导，提示免疫抑制剂作用于B细胞区室的一种机制。我们提出，NF-AT应更普遍地被视为活化淋巴细胞的核因子。
