Mori M, Barnard G F, Mimori K, Ueo H, Akiyoshi T, Sugimachi K
Department of Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1995 Mar 15;75(6 Suppl):1516-9. doi: 10.1002/1097-0142(19950315)75:6+<1516::aid-cncr2820751522>3.0.co;2-7.
Matrix metalloproteinase-7 (MMP-7) is a member of the family of matrix-degrading metalloproteinases (MMPs) that are considered to contribute to cancer invasion and metastasis. One of the gene products that the authors have isolated by a cDNA subtraction of human gastric cancer minus normal gastric mucosa is MMP-7. The purpose of this study was to examine the expression of MMP-7 mRNA in different stages of human colorectal carcinomas.
A subtracted complementary DNA library was generated from a paired sample of human gastric carcinoma minus normal gastric mucosa. Screening of the library clones determined that MMP-7 mRNA was overexpressed. The complementary DNA insert was then used as a probe to evaluate the expression in a larger number of surgical samples of human colorectal carcinomas by Northern hybridization.
The mRNA signal was greater in the colorectal carcinoma than in paired adjacent normal colonic or rectal mucosa in 39 of 47 cases. The expression of MMP-7 mRNA in tumor tissues increased with increasing Dukes' stage (P < 0.05). The MMP-7 mRNA expression was greatest in the metastatic liver lesions.
The findings imply that MMP-7 mRNA is overexpressed in human colorectal carcinomas and that MMP-7 may prove useful as a marker of biologic aggressiveness.
基质金属蛋白酶-7（MMP-7）是基质降解金属蛋白酶（MMPs）家族的成员之一，被认为与癌症侵袭和转移有关。作者通过人胃癌减去正常胃黏膜的cDNA消减杂交分离出的基因产物之一就是MMP-7。本研究的目的是检测MMP-7 mRNA在人结直肠癌不同阶段的表达情况。
从人胃癌减去正常胃黏膜的配对样本中构建消减互补DNA文库。对文库克隆进行筛选，确定MMP-7 mRNA过度表达。然后将互补DNA插入片段用作探针，通过Northern杂交评估大量人结直肠癌手术样本中的表达情况。
47例中有39例结直肠癌中的mRNA信号比配对的相邻正常结肠或直肠黏膜中的信号更强。肿瘤组织中MMP-7 mRNA的表达随着Dukes分期的增加而升高（P < 0.05）。MMP-7 mRNA在转移性肝病变中的表达最高。
这些发现表明MMP-7 mRNA在人结直肠癌中过度表达，并且MMP-7可能被证明是生物学侵袭性的一个有用标志物。
