Carsenti-Etesse H, Durant J, De Salvador F, Bensoussan M, Bensoussan F, Pradier C, Bernard E, Mondain V, Thabaut A, Dellamonica P
Archet Hospital, Nice University, France.
Eur J Clin Microbiol Infect Dis. 1994 Dec;13(12):1058-62. doi: 10.1007/BF02111828.
The development of resistance in vitro in five strains of Streptococcus pneumoniae (3 with full susceptibility and 2 with intermediate susceptibility to penicillin) was investigated by serial passages in the presence of subinhibitory concentrations of amoxicillin and ampicillin. At the end of passaging, MICs of antibiotics for all the strains increased by a factor of four or more, reaching at least intermediate levels. MICs of cephalosporins, ampicillin and amoxicillin increased for almost all variants obtained. Similar results were obtained with amoxicillin plus clavulanic acid at a ratio of 2:1 and at a constant concentration of 2 micrograms/ml, and with ampicillin plus sulbactam at a ratio 2:1. In contrast, no significant modification of MIC was seen with ampicillin plus sulbactam at a constant concentration of 4 micrograms/ml sulbactam. These results suggest interaction of sulbactam with penicillin binding proteins as described previously for other bacterial species, and merit further investigation.
通过在亚抑制浓度的阿莫西林和氨苄西林存在下进行连续传代，研究了五株肺炎链球菌（3株对青霉素完全敏感，2株对青霉素中度敏感）的体外耐药性发展情况。传代结束时，所有菌株对抗生素的最低抑菌浓度（MIC）增加了四倍或更多，达到至少中度水平。几乎所有获得的变体的头孢菌素、氨苄西林和阿莫西林的MIC均增加。以2:1的比例和2微克/毫升的恒定浓度使用阿莫西林加克拉维酸，以及以2:1的比例使用氨苄西林加舒巴坦，也得到了类似的结果。相比之下，在舒巴坦恒定浓度为4微克/毫升的情况下，使用氨苄西林加舒巴坦未观察到MIC有显著变化。这些结果表明舒巴坦与青霉素结合蛋白存在相互作用，如先前针对其他细菌物种所描述的那样，值得进一步研究。
