Weathersbee P S, Johnson A R
Irvine Scientific, Santa Ana, California.
Fertil Steril. 1995 Apr;63(4):925-8. doi: 10.1016/s0015-0282(16)57505-0.
To determine whether modified TES and Tris (TEST) yolk buffer (TYB) made using a commercially available egg yolk extract would exhibit lab performance characteristics equal to the existing preparation made with whole egg yolk and to define the phospholipid content of the new modified TYB formulation.
Divided ejaculates from 21 normozoospermic and 7 oligozoospermic males presenting for pre-IVF evaluation were stored at 0 to 4 degrees C for 42 hours using commercially available or modified TYB before analysis in the optimized sperm penetration assay (SPA).
A commercial tissue culture manufacturer and a clinical fertility reference laboratory.
Sperm swim-up recoveries and average penetrations per ovum, determined by the SPA, were used as measures of sperm function. High-pressure liquid chromatography (HPLC) was used to profile the egg yolk extract.
No significant differences in either sperm swim-up recovery rates or SPA results were found in normal or poor quality semen that was treated with modified or commercial TYB. The major constituent in commercial egg yolk extract is lecithin.
Commercially available egg yolk extract passes easily through a 0.2-microns filter, is a rich source of lecithin, and can be substituted effectively for whole egg yolk in preparing TYB.
确定使用市售蛋黄提取物制成的改良三乙醇胺（TES）和三羟甲基氨基甲烷（Tris）（TEST）蛋黄缓冲液（TYB）是否具有与用全蛋黄制成的现有制剂相同的实验室性能特征，并确定新改良TYB制剂的磷脂含量。
将21名正常精子症男性和7名少精子症男性在进行体外受精前评估时的精液标本分为两份，分别使用市售或改良的TYB在0至4摄氏度下保存42小时，然后在优化的精子穿透试验（SPA）中进行分析。
一家商业组织培养制造商和一家临床生育参考实验室。
通过SPA测定的精子上游回收率和每个卵子的平均穿透率用作精子功能的指标。使用高压液相色谱（HPLC）对蛋黄提取物进行分析。
在用改良或市售TYB处理的正常或质量较差的精液中，精子上游回收率或SPA结果均未发现显著差异。市售蛋黄提取物的主要成分是卵磷脂。
市售蛋黄提取物易于通过0.2微米的过滤器，是卵磷脂的丰富来源，并且在制备TYB时可以有效地替代全蛋黄。
