Standish A, Enquist L W, Escardo J A, Schwaber J S
Neural Computation Group, E. I. DuPont de Nemours and Company, Wilmington, Delaware 19880-0328.
J Neurosci. 1995 Mar;15(3 Pt 1):1998-2012. doi: 10.1523/JNEUROSCI.15-03-01998.1995.
We defined the central circuit innervating various regions of the rat heart using a neurotropic herpesvirus as a transneuronal tracer. Location of viral antigens in the brain after cardiac injection of three strains of pseudorabies virus (PRV) provided insight into vagal preganglionic neurons and their connected interneurons. At short survival times, labeled vagal preganglionic neurons were localized in both the nucleus ambiguus (NA) and the dorsal motor nucleus of the vagus (DMV), and in an arc-like band through the reticular formation between the NA and the DMV. The amount of DMV labeling was dependent on viral strain. Similar distributions of labeled neurons were observed following either ganglionic, sinoatrial node, or ventricular injections. At intermediate survival times postcardiac injection, the virus replicated in vagal preganglionic neurons and was trans-synaptically transported to interneurons observed primarily in the NA regions and in an arc-like band through the reticular formation. Labeled neurons were also observed in ventral regions of the nucleus of the solitary tract (NTS). At longer survival times, labeled neurons were found in various regions of the NTS with the most abundant label dorsal and dorsomedial to the solitary tract. Abundant neuronal labeling was also found in the intermediolateral cell column, the raphe nuclei, the caudal and rostral ventral lateral medulla, the A5 region, the locus coeruleus, and the lateral and paraventricular hypothalamic nuclei. These data define the central circuits including the interneuronal connections that innervate various cardiac targets.
我们使用嗜神经疱疹病毒作为跨神经元示踪剂，定义了支配大鼠心脏各个区域的中枢回路。心脏注射三株伪狂犬病病毒（PRV）后，病毒抗原在大脑中的定位为迷走神经节前神经元及其相连的中间神经元提供了深入了解。在短存活时间内，标记的迷走神经节前神经元定位于疑核（NA）和迷走神经背运动核（DMV），以及通过NA和DMV之间的网状结构形成的弧形带。DMV标记的数量取决于病毒株。在神经节、窦房结或心室注射后，观察到标记神经元的分布相似。在心脏注射后的中间存活时间，病毒在迷走神经节前神经元中复制，并通过突触转运到主要在NA区域和通过网状结构的弧形带中观察到的中间神经元。在孤束核（NTS）的腹侧区域也观察到标记神经元。在更长的存活时间，在NTS的各个区域发现了标记神经元，在孤束背侧和背内侧标记最为丰富。在中间外侧细胞柱、中缝核、尾侧和头侧腹外侧延髓、A5区域、蓝斑以及外侧和室旁下丘脑核中也发现了丰富的神经元标记。这些数据定义了包括支配各种心脏靶点的中间神经元连接在内的中枢回路。
