Dusart I, Morel M P, Sotelo C
INSERM U106, Hôpital de la Salpétrière, Paris, France.
Neuroscience. 1994 Nov;63(2):351-6. doi: 10.1016/0306-4522(94)90534-7.
The pattern of expression of p75, the low affinity nerve growth factor receptor, in the adult rat cerebellum and its fate after a traumatic lesion were analysed using immunohistochemical localization of this receptor. A subset of Purkinje cells was immunoreactive for low affinity nerve growth factor receptor in the intact adult cerebellum. These cells were arranged in alternating positive and negative parasagittal compartments along the cerebellar cortex. This pattern of expression had 90% homology with zebrin I. After a traumatic lesion, the specific pattern of expression of zebrin I remained unchanged, whereas the low affinity nerve growth factor receptor pattern changed as early as one day: Purkinje cells near the lesion site, independent of zebrin I staining, became immunoreactive. During the first week, the increase in immunoreactivity remained high. Thereafter, there was a short, fast decrease followed by a long period in which a faint immunostaining on lesioned Purkinje cells is maintained for up to one year. The increase in the expression of the low affinity nerve growth factor receptor by all traumatically affected Purkinje cells suggests a correlation between this specific up-regulation and the high resistance of these neurons to axotomy or other traumatic injuries.
利用低亲和力神经生长因子受体的免疫组织化学定位，分析了成年大鼠小脑中该受体的表达模式及其在创伤性损伤后的变化情况。在完整的成年小脑中，一部分浦肯野细胞对低亲和力神经生长因子受体呈免疫反应阳性。这些细胞沿小脑皮质以正负相间的矢状旁区排列。这种表达模式与斑马蛋白I有90%的同源性。创伤性损伤后，斑马蛋白I的特异性表达模式保持不变，而低亲和力神经生长因子受体模式早在一天后就发生了变化：损伤部位附近的浦肯野细胞，无论斑马蛋白I染色情况如何，都变得具有免疫反应性。在第一周内，免疫反应性持续大幅增加。此后，出现短暂、快速的下降，随后是一个较长的时期，在此期间，损伤的浦肯野细胞上的微弱免疫染色可持续长达一年。所有受创伤影响的浦肯野细胞中低亲和力神经生长因子受体表达的增加表明，这种特定的上调与这些神经元对轴突切断或其他创伤性损伤的高抗性之间存在关联。
