Dietzler D N, Leckie M P, Crimmins D L, Ungar J M, Sternheim W L
J Bacteriol. 1976 Oct;128(1):165-9. doi: 10.1128/jb.128.1.165-169.1976.
A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equation was derived from empirical equations that describe the relationship between the rate of glucose utilization and one of these hexose phosphates in metabolic situations where the other hexoses phosphate was not altered. In the experiments described in this report, treatment of nitrogen (NH4+)-starved cultures of E. coli W4597 (K) with various concentrations of sodium azide altered the levels of both hexose phosphates as well as the rate of glucose utilization. In each case the observed rate and the rate predicted by the comprehensive equation agreed closely, substantiating the validity of this comprehensive relationship as a quantitative indicator of metabolic events in the intact cell. The mechanism of metabolic regulation that is represented by this equation is discussed in light of the cellular levels of adenosine 5'-triphosphate and phosphoenolpyruvate observed in these experiments.
有人提出一个综合方程式，即 υ = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)]，用于表示完整大肠杆菌细胞中葡萄糖利用速率与葡萄糖-6-磷酸和果糖-1,6-二磷酸水平之间的定量相互关系。这个综合方程式源自经验方程式，这些经验方程式描述了在另一种己糖磷酸未改变的代谢情况下，葡萄糖利用速率与这些己糖磷酸之一之间的关系。在本报告所述的实验中，用不同浓度的叠氮化钠处理大肠杆菌W4597（K）的氮（NH4+）饥饿培养物，改变了两种己糖磷酸的水平以及葡萄糖利用速率。在每种情况下，观察到的速率与综合方程式预测的速率都非常吻合，证实了这种综合关系作为完整细胞中代谢事件定量指标的有效性。根据这些实验中观察到的细胞腺苷5'-三磷酸和磷酸烯醇丙酮酸水平，讨论了由该方程式表示的代谢调节机制。
