Szende B, Csikós A, Szepeshazi K, Neill J D, Mulchahey J J, Halmos G, Lapis K, Schally A V
Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary.
Receptor. 1994 Winter;4(4):201-7.
LH-RH analogs cause some inhibition of growth of pancreatic cancers. Syrian golden hamsters bearing chemically induced pancreatic cancers were treated with [D-Trp6]LH-RH for 3 d before sacrifice. LH-RH receptors were localized by electron-microscopic immunohistochemistry in the tumor cells of both treated and untreated hamsters. [D-Trp6]LH-RH treatment resulted in a marked increase in the concentration of LH-RH receptors in the nuclei. The dissociation constants (Kd) and the maximal binding capacity of the LH-RH receptors (Bmax), measured by radioreceptor assay, were higher in the nuclei of the pancreatic tumor cells of hamsters treated with [D-Trp6]LH-RH than in the untreated controls. Pancreatic cells of tumor-free hamsters did not show immunostaining for LH-RH receptors. A possible correlation between the increase in the concentration of the LH-RH receptors in the nuclei and the tumor growth-inhibiting activity of [D-Trp6]LH-RH is suggested.
促黄体生成素释放激素（LH-RH）类似物对胰腺癌的生长有一定抑制作用。对携带化学诱导胰腺癌的叙利亚金黄地鼠在处死前用[D-色氨酸6]LH-RH处理3天。通过电子显微镜免疫组织化学法在经处理和未经处理的地鼠肿瘤细胞中定位LH-RH受体。[D-色氨酸6]LH-RH处理导致细胞核中LH-RH受体浓度显著增加。通过放射受体分析法测得，经[D-色氨酸6]LH-RH处理的地鼠胰腺肿瘤细胞核中LH-RH受体的解离常数（Kd）和最大结合容量（Bmax）高于未处理的对照组。无肿瘤地鼠的胰腺细胞未显示出LH-RH受体的免疫染色。提示细胞核中LH-RH受体浓度的增加与[D-色氨酸6]LH-RH的肿瘤生长抑制活性之间可能存在相关性。
