Angell S M, Baulcombe D C
Sainsbury Laboratory, Norwich Research Park, Colney, UK.
Plant J. 1995 Jan;7(1):135-40. doi: 10.1046/j.1365-313x.1995.07010135.x.
The replication and cell-to-cell movement of potato virus X (PVX) has been studied using a PVX vector construct which expressed the beta-glucuronidase (GUS) reporter gene in infected cells. Nicotiana clevelandii leaf trichome cells were micro-injected with the PVX-GUS vector and histochemical staining was used to locate GUS activity. The distribution of GUS activity revealed that PVX had moved from the injected cell into other trichome cells and into the cells along the leaf margin. GUS activity was always restricted to the cells at the edge of the leaf suggesting that PVX was unable to move out of the marginal cells. At the infection front, scattered along the leaf margin, there were isolated groups of cells staining for GUS activity. The absence of GUS activity in the intervening cells suggests that PVX had moved through several cells without replicating within them. This latter observation is consistent with previously reported observations that viral movement proteins are capable of moving between cells.
利用一种在受感染细胞中表达β-葡萄糖醛酸酶（GUS）报告基因的马铃薯X病毒（PVX）载体构建体，对PVX的复制及细胞间运动进行了研究。用PVX-GUS载体对克利夫兰烟草叶毛细胞进行显微注射，并采用组织化学染色来定位GUS活性。GUS活性的分布表明，PVX已从注射细胞转移到其他毛细胞以及沿叶缘的细胞中。GUS活性始终局限于叶边缘的细胞，这表明PVX无法从边缘细胞中移出。在感染前沿，沿叶缘分散分布着孤立的GUS活性染色细胞群。中间细胞中缺乏GUS活性表明，PVX在不于其中复制的情况下穿过了几个细胞。后一观察结果与先前报道的病毒运动蛋白能够在细胞间移动的观察结果一致。
