Saito A, Yoshii Y, Nose T
Department of Neurosurgery, University of Tsukuba.
Noshuyo Byori. 1994;11(2):143-6.
In order to estimate the degree of malignancy of astrocytomas, we applied image analysis to study the nuclear DNA content and morphometric parameters of individual tumor cells in surgically resected specimens of 20 patients. There were nine low grade astrocytomas (LGA) and 11 high grade astrocytomas (HGA). Although the mean DNA index did not indicate a significant difference between LGA and HGA, five (45%) of the 11 HGA had an aneuploid DNA distribution pattern. By contrast, all LGA had a diploid pattern. The ratio of the S-G2M phase (% S-G2M) of the HGA (17.5 +/- 12.3%) was significantly higher than that of the LGA (7.0 +/- 4.6%). From the morphometric analysis of the area and shape of the nucleus of tumor cells, we obtained quantitative estimates of the degree of nuclear pleomorphism in astrocytomas. The nuclei of the tumor cells of HGA had larger sizes, increased anisokaryosis and were more irregular. In contrast to flow cytometry, image analysis can be used for quantitative studies of morphological tumor cell characteristics of relevance in the grading of malignancy.
为了评估星形细胞瘤的恶性程度，我们应用图像分析技术研究了20例手术切除标本中单个肿瘤细胞的核DNA含量和形态计量学参数。其中有9例低级别星形细胞瘤（LGA）和11例高级别星形细胞瘤（HGA）。虽然平均DNA指数在LGA和HGA之间未显示出显著差异，但11例HGA中有5例（45%）具有非整倍体DNA分布模式。相比之下，所有LGA均为二倍体模式。HGA的S-G2M期比例（% S-G2M，17.5 +/- 12.3%）显著高于LGA（7.0 +/- 4.6%）。通过对肿瘤细胞核的面积和形状进行形态计量学分析，我们获得了星形细胞瘤核多形性程度的定量评估。HGA肿瘤细胞的核更大，核大小不等增加且更不规则。与流式细胞术不同，图像分析可用于对肿瘤细胞形态特征进行定量研究，这些特征与恶性程度分级相关。
