Enhanced albumin protein separations and protein-drug binding constant measurements using anti-inflammatory drugs as run buffer additives in affinity capillary electrophoresis.

An affinity capillary electrophoresis (ACE) method for improving albumin protein separations has been developed. Separation efficiencies for bovine serum albumin (BSA) and human serum albumin (HSA) are dramatically improved by using anti-inflammatory compounds as run buffer additives. The anti-inflammatory drugs used as biospecific ligands to improve the protein separation include ibuprofen (IB), flurbiprofen (FL), and ketoprofen (KE). The binding constants of proteins (BSA and HSA) for the anti-inflammatory ligands (FL and IB) are estimated by ACE and compared to literature values.