通过体内全细胞记录揭示的青蛙视网膜神经节细胞与顶盖神经元之间的突触连接模式。

Synaptic connection patterns between frog retinal ganglion cells and tectal neurons revealed by whole-cell recordings in vivo.

作者信息

Nakagawa H, Kikkawa S, Matsumoto N

机构信息

Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.

出版信息

Brain Res. 1994 Dec 5;665(2):319-22. doi: 10.1016/0006-8993(94)91355-2.

DOI:10.1016/0006-8993(94)91355-2

PMID:7895070

Abstract

The synaptic connections between frog retinal ganglion cells and tectal neurons were examined by whole-cell recordings in vivo combined with nystatin perforated patch-clamp technique and pulse-triggered averaging analysis. In five tectal neurons, monosynaptic EPSPs from seven R3 retinal ganglion cells were observed. One tectal neuron was found to receive EPSPs from both R1/2 and R3 retinal ganglion cell simultaneously. Thus we could provide the first evidence for convergence of multiple types of retinal axons upon a single tectal neuron.

摘要

采用体内全细胞记录结合制霉菌素穿孔膜片钳技术和脉冲触发平均分析，研究了青蛙视网膜神经节细胞与顶盖神经元之间的突触连接。在五个顶盖神经元中，观察到来自七个R3视网膜神经节细胞的单突触兴奋性突触后电位（EPSP）。发现一个顶盖神经元同时接受来自R1/2和R3视网膜神经节细胞的EPSP。因此，我们能够提供首个证据，证明多种类型的视网膜轴突会聚于单个顶盖神经元。