Yeh J Y, Beilstein M A, Andrews J S, Whanger P D
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
FASEB J. 1995 Mar;9(5):392-6. doi: 10.1096/fasebj.9.5.7896009.
Rabbits were immunized with two synthetic peptides based on hydrophilic regions of selenoprotein W from rat muscle. The resulting polyclonal antibodies were used in Western blots to determine the compartmentation and tissue distribution of selenoprotein W, and to determine the influence of selenium on the levels of this selenoprotein in rat muscle. Selenoprotein W exists mainly in cytosol, but very small amounts were associated with membranes. Western blots revealed selenoprotein W in muscle, spleen, testis, and brain of rats. Rats were fed diets of either no addition of selenium (0 ppm Se) or additions of 0.1 and 4.0 mg selenium/g (0.1 ppm Se and 4.0 ppm Se) diet for 6 wk. Selenoprotein W was undetectable in skeletal muscle of rats fed the basal diet, detectable in those fed 0.1 ppm selenium in the diet, and much higher in muscle from rats fed 4 ppm selenium diet. In a species comparison, Western blots indicated the presence of selenoprotein W in muscle of rabbits, sheep, and cattle.
用基于大鼠肌肉硒蛋白W亲水区域的两种合成肽对兔子进行免疫。所得多克隆抗体用于蛋白质免疫印迹法，以确定硒蛋白W的区室化和组织分布，并确定硒对大鼠肌肉中这种硒蛋白水平的影响。硒蛋白W主要存在于细胞质中，但与细胞膜相关的量非常少。蛋白质免疫印迹法显示大鼠的肌肉、脾脏、睾丸和大脑中存在硒蛋白W。给大鼠喂食不添加硒（0 ppm硒）或添加0.1和4.0毫克硒/克（0.1 ppm硒和4.0 ppm硒）的饲料，持续6周。喂食基础饲料的大鼠骨骼肌中未检测到硒蛋白W，喂食含0.1 ppm硒饲料的大鼠中可检测到，而喂食含4 ppm硒饲料的大鼠肌肉中硒蛋白W含量更高。在物种比较中，蛋白质免疫印迹法表明兔子、绵羊和牛的肌肉中存在硒蛋白W。
