LY 83583和亚甲蓝对一氧化氮、硝酸甘油、硝普钠及心房肽在大鼠、豚鼠和家兔主动脉中所诱导舒张作用的影响

The relaxation by nitroglycerin (GTN) and nitric oxide (NO) of aortic smooth muscles from rabbit and rat contracted by phenylephrine was inhibited by LY 83583 (LY) and methylene blue (MB) (the same applied to guinea-pig aorta), while the relaxation by SNP was not inhibited in rabbit. The relaxation by ANP was not inhibited. 2. All these agents produced concentration-dependent increases in cyclic GMP. While the increases by GTN and NO were inhibited by LY and MB, the increases by SNP were inhibited only in rat and those by ANP were not inhibited. 3. Thus, LY behaved essentially similar to MB, indicating that the substance is an inhibitor of activation of soluble guanylate cyclase by NO and NO-related vasodilators. It was assumed that, like MB, LY facilitated intracellular release of NO from SNP in rabbit.

硝酸甘油（GTN）和一氧化氮（NO）对苯肾上腺素收缩的兔和大鼠主动脉平滑肌的舒张作用，被LY 83583（LY）和亚甲蓝（MB）抑制（豚鼠主动脉也如此），而硝普钠（SNP）对兔主动脉平滑肌的舒张作用未被抑制。心房钠尿肽（ANP）的舒张作用未被抑制。2. 所有这些药物均使环磷酸鸟苷（cGMP）浓度依赖性增加。虽然GTN和NO引起的cGMP增加被LY和MB抑制，但SNP引起的cGMP增加仅在大鼠中被抑制，而ANP引起的cGMP增加未被抑制。3. 因此，LY的作用与MB基本相似，表明该物质是NO及NO相关血管舒张剂激活可溶性鸟苷酸环化酶的抑制剂。据推测，与MB一样，LY促进了兔体内SNP的NO细胞内释放。

Modification by LY 83583 and methylene blue of relaxation induced by nitric oxide, glyceryl trinitrate, sodium nitroprusside and atriopeptin in aortae of the rat, guinea-pig and rabbit.