Igarashi Y, Goldrich M S, Kaliner M A, Irani A M, Schwartz L B, White M V
Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
J Allergy Clin Immunol. 1995 Mar;95(3):716-25. doi: 10.1016/s0091-6749(95)70177-x.
The role of inflammatory cells at the local site of allergic inflammation in the nose is unclear.
Nasal biopsy specimens were obtained from 10 patients with symptomatic seasonal allergic rhinitis and 10 normal subjects. Freeze-dried paraffin-embedded sections were stained for mononuclear cells and eosinophils. Tissues in Carnoy's fixative were stained for mast cells.
T cells were much more plentiful than B cells or macrophages, and no significant differences were found between the two groups in the number of T cells, T-cell subsets, B cells, and macrophages. However, the number of CD25+ cells (lymphocyte activation markers) and the number of eosinophils were significantly higher in the allergic group than in the control group. There were no significant differences between the two groups in the total mast cell number. However, mucosal type mast cells were slightly increased, and a higher ratio of mast cells were costained for IgE in the allergic group. IgE+ cells mostly constained for mast cell tryptase and did not costain for J chain.
These data suggest that unlike granulocytes, in some mononuclear cells qualitative, not quantitative, changes may be important in allergic rhinitis and that IgE may not be locally produced in the nasal mucosa.
炎症细胞在鼻部过敏性炎症局部部位的作用尚不清楚。
从10例有症状的季节性变应性鼻炎患者和10名正常受试者获取鼻活检标本。对冻干石蜡包埋切片进行单核细胞和嗜酸性粒细胞染色。用卡诺固定液固定的组织进行肥大细胞染色。
T细胞比B细胞或巨噬细胞丰富得多，两组在T细胞、T细胞亚群、B细胞和巨噬细胞数量上无显著差异。然而，变应性组CD25 +细胞（淋巴细胞活化标志物）数量和嗜酸性粒细胞数量显著高于对照组。两组肥大细胞总数无显著差异。然而，黏膜型肥大细胞略有增加，变应性组中IgE共染色的肥大细胞比例更高。IgE +细胞大多与肥大细胞类胰蛋白酶共染色，不与J链共染色。
这些数据表明，与粒细胞不同，在某些单核细胞中，质量而非数量的变化在变应性鼻炎中可能很重要，并且IgE可能不是在鼻黏膜局部产生的。
