Escalante R, García-Sáez A, Sastre L
Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Científicas, Madrid, Spain.
J Histochem Cytochem. 1995 Apr;43(4):391-9. doi: 10.1177/43.4.7897181.
The spatial pattern of expression of the mRNA encoded by the Na,K-ATPase alpha-subunit cDNA clone pArATNa136 was determined by in situ hybridization of first, second, and third instar Artemia franciscana larvae. This mRNA was expressed at high levels in the salt gland, the antennal gland, and the end of the midgut, which are the three main osmoregulatory organs in Artemia at these stages of development. The pattern of expression was similar at the three stages of development analyzed, although the level of expression increased during development, especially in the salt and antennal glands. The expression of the mRNA coding for another Na, K-ATPase alpha-subunit isoform, the proposed alpha 2-isoform, was also determined and was shown to be limited to the salt gland. These results suggest that the clone pArATNa136 codes for the biochemically defined alpha 1-isoform of the Na,K-ATPase alpha-subunit and reinforce the importance of this isoform in osmoregulation at the three larval stages studied. The alpha 2-isoform may also be involved in osmoregulation during the first stages of larval development.
通过对卤虫（Artemia franciscana）一龄、二龄和三龄幼虫进行原位杂交，确定了由钠钾ATP酶α亚基cDNA克隆pArATNa136编码的mRNA的空间表达模式。在盐腺、触角腺和中肠末端，这种mRNA高水平表达，在卤虫发育的这些阶段，这三个部位是主要的渗透调节器官。在所分析的三个发育阶段，表达模式相似，尽管在发育过程中表达水平增加，尤其是在盐腺和触角腺。另一种钠钾ATP酶α亚基异构体（推测为α2异构体）编码的mRNA的表达也被确定，并且显示仅限于盐腺。这些结果表明，克隆pArATNa136编码钠钾ATP酶α亚基生化定义的α1异构体，并强化了该异构体在所研究的三个幼虫阶段渗透调节中的重要性。α2异构体可能在幼虫发育的最初阶段也参与渗透调节。
