Shionoya K
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1994 Dec;61(4):580-9. doi: 10.5357/koubyou.61.580.
Transferrin receptor (TfR) is expressed in the proliferating cells. The author performed an immunohistochemical investigation with TfR and an analysis of TfR protein and mRNA in several cell-lines and examined the relationship between the TfR content and the proliferative activity of the tumor cells. Immunohistochemical examination revealed a strong reaction of the TfR was found in the cell membrane of the epithelial malignant tumors and the non-epithelial malignant tumors showed diffuse staining in the cytoplasm. TfR was almost regulated by the cellular density in the culture dishes. A confluent phase growth led to a low level of TfR. And among the several cell-lines, a higher level of TfR was seen in the cell of the short-doubling time than that of the long-doubling time. In vivo, similarly the rapid growth tumor indicated a high level of TfR rather than the slow growth one. Thus, the level of TfR indicates the degree of the proliferative activity of the tumor cells, so the examination of TfR content is helpful for the determination of the degree of malignancy in each tumor case.
转铁蛋白受体（TfR）在增殖细胞中表达。作者用TfR进行了免疫组织化学研究，并对几种细胞系中的TfR蛋白和mRNA进行了分析，还研究了TfR含量与肿瘤细胞增殖活性之间的关系。免疫组织化学检查显示，在上皮性恶性肿瘤的细胞膜中发现TfR有强烈反应，而非上皮性恶性肿瘤在细胞质中呈弥漫性染色。TfR几乎受培养皿中细胞密度的调节。汇合期生长导致TfR水平较低。在几种细胞系中，短倍增时间的细胞比长倍增时间的细胞中TfR水平更高。在体内，同样快速生长的肿瘤比生长缓慢的肿瘤TfR水平更高。因此，TfR水平表明肿瘤细胞的增殖活性程度，所以检测TfR含量有助于确定每个肿瘤病例的恶性程度。
