Oren A, Taylor J M
UCLA School of Medicine, Department of Medicine, 90024-1690.
J Lab Clin Med. 1995 Mar;125(3):340-7.
We used immunogold electron microscopy to examine the distribution of human defensins 1-3 (HNP1-3) and myeloperoxidase (MPO) in the cytoplasmic granules of thin-sectioned, normal human neutrophils. The presence of MPO, defensins, or both was noted in 388 (62.3%) of 623 granule profiles. Whereas MPO and defensins colocalized in 32.5% of such granules, 32.7% demonstrated only MPO and 34.8% demonstrated only defensins. The staining densities of MPO and HNP1-3 showed a low correlation, even when both molecules were present in the same granules (r = 0.09), indicating that they were not present in fixed relative proportions even when colocalized. The low partial correlation coefficient (r = -0.23) between MPO and HNP1-3 for the 388 labeled granules controlling for granule area suggested that the deposition of MPO and defensins might even be negatively correlated. The distribution of MPO and defensins within the cytoplasmic granules of human neutrophils followed a "non-colocalization and multi-compartmental" model that conflicts with the classical notion of azurophil granules as a homogeneous population of organelles marked by the presence of MPO.
我们采用免疫金电子显微镜技术，检测正常人中性粒细胞薄切片胞质颗粒中人防御素1 - 3（HNP1 - 3）和髓过氧化物酶（MPO）的分布。在623个颗粒断面中，有388个（62.3%）观察到MPO、防御素或两者均存在。MPO和防御素在32.5%的此类颗粒中共定位，32.7%仅显示MPO，34.8%仅显示防御素。即使MPO和HNP1 - 3存在于同一颗粒中，它们的染色密度相关性也较低（r = 0.09），这表明即使共定位，它们也并非以固定的相对比例存在。在控制颗粒面积的情况下，388个标记颗粒中MPO与HNP1 - 3的偏相关系数较低（r = -0.23），提示MPO和防御素的沉积甚至可能呈负相关。人中性粒细胞胞质颗粒中MPO和防御素的分布遵循“非共定位和多区室”模型，这与嗜天青颗粒作为以MPO存在为特征的均匀细胞器群体的经典概念相矛盾。
