Sánchez-Lanier M, Triplett C, Campion M
Department of Microbiology, Washington State University, Pullman 99164-4233.
J Med Virol. 1994 Dec;44(4):369-78. doi: 10.1002/jmv.1890440410.
There are about 20 types of human papillomaviruses (HPVs) which are generally believed to be confined to the anogenital tract. HPV 16 is the most common of these. Four squamous cell carcinomas of the finger from three individuals were analyzed for the presence of HPV DNA by Pst 1 restriction enzyme digest and Southern blotting. Under high stringency conditions, all were positive for HPV 16. The DNA from each patient was digested with a panel of restriction enzymes so that integration of HPV into the genome could be evaluated. Three of three samples contained high molecular weight oligomeric circular DNA arranged as catanates. None showed integration. To determine if the HPV was transcriptionally active, RNA was isolated, reverse transcribed, and amplified using primers that amplified the unspliced E6 transcript and the E6I and E6II spliced transcripts. All of the patient biopsies assayed expressed the unspliced E6 transcript and the spliced E6I transcript with the E6I transcript being the most abundant. The E6II transcript was not detected in any of the samples. These findings indicate that HPV plays a role in the development of squamous cell carcinoma of the finger and the role of the malignant genital HPV needs to be carefully looked at in areas outside the genital region. It also suggests that the natural history of HPV in areas outside the cervix may not be identical to that of HPV in the anogenital region.
大约有20种人乳头瘤病毒（HPV），一般认为它们局限于肛门生殖道。HPV 16是其中最常见的。对来自三名个体的四例手指鳞状细胞癌进行了分析，通过Pst 1限制性内切酶消化和Southern印迹法检测HPV DNA的存在。在高严格条件下，所有样本HPV 16均呈阳性。用一组限制性内切酶消化每位患者的DNA，以便评估HPV整合到基因组中的情况。三个样本中的三个含有排列成链状的高分子量寡聚环状DNA。没有一个显示整合。为了确定HPV是否具有转录活性，提取RNA，进行逆转录，并使用扩增未剪接的E6转录本以及E6I和E6II剪接转录本的引物进行扩增。所有检测的患者活检样本均表达未剪接的E6转录本和剪接的E6I转录本，其中E6I转录本最为丰富。在任何样本中均未检测到E6II转录本。这些发现表明HPV在手指鳞状细胞癌的发生中起作用，并且需要在生殖器区域以外的部位仔细研究恶性生殖器HPV的作用。这也表明子宫颈以外部位的HPV自然史可能与肛门生殖道区域的HPV不同。
