Georgakopoulos T, Gounalaki N, Thireos G
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece.
Mol Gen Genet. 1995 Mar 20;246(6):723-8. doi: 10.1007/BF00290718.
The GCN5 and ADA2 proteins are required for the activation function of a number of transcriptional activators in the yeast Saccharomyces cerevisiae. By using appropriate LexA fusion proteins we demonstrated that both proteins are required for part of the function of the GCN4, GAL4 and the VP16 transcriptional activation domains. Analysis of a gcn5 ada2 double disruption mutant did not reveal any additive effects, suggesting that the two proteins act in the same pathway. The GCN5 and ADA2 proteins can each activate transcription when directed to the promoter region of a reporter gene, but only in the presence of a wild-type ADA2 or GCN5 gene, respectively. The activation capacity is enhanced when the corresponding endogenous gene copy is disrupted. Taken together, these genetic data suggest that the two proteins interact and define one complex that mediates transcriptional activation. The function of this complex requires the bromodomain region of the GCN5 protein.
GCN5和ADA2蛋白是酿酒酵母中许多转录激活因子激活功能所必需的。通过使用合适的LexA融合蛋白，我们证明这两种蛋白是GCN4、GAL4和VP16转录激活域部分功能所必需的。对gcn5 ada2双缺失突变体的分析未发现任何累加效应，表明这两种蛋白在同一途径中起作用。当分别导向报告基因的启动子区域时，GCN5和ADA2蛋白各自都能激活转录，但分别仅在存在野生型ADA2或GCN5基因的情况下。当相应的内源性基因拷贝被破坏时，激活能力增强。综上所述，这些遗传学数据表明这两种蛋白相互作用并定义了一个介导转录激活的复合物。该复合物的功能需要GCN5蛋白的溴结构域区域。
