Van Voorst A, Leenstra F R
Institute for Animal Science and Health (ID-DLO) Het Spelderholt, Beekbergen, The Netherlands.
Poult Sci. 1995 Jan;74(1):136-40. doi: 10.3382/ps.0740136.
Semen was collected for 4 consecutive d individually from experimental broiler breeder males that had not been massaged for 7 d. The semen was mixed and diluted with the Beltsville Poultry Semen Extender with dimethylsulfoxide (DMSO) as cryoprotectant and cryopreserved. After thawing of the semen, hens were inseminated and fertility over 1 wk after a single insemination with the cryopreserved semen was determined. The overall fertility rate in this experiment (83 to 93%) was high, compared with the generally reported fertility rate of frozen-thawed fowl semen. The fertility rate of the semen collected on Days 3 and 4 was significantly higher than that of semen collected on Day 1 or 2, indicating that frequent collection over the 4-d period enhances the fertility rate of the semen.
从连续7天未进行按摩的实验用肉种公鸡个体中，每天单独采集精液，持续4天。采集的精液混合后，用含有二甲亚砜（DMSO）作为冷冻保护剂的贝尔茨维尔家禽精液稀释液进行稀释并冷冻保存。精液解冻后，对母鸡进行输精，并测定单次输精冷冻保存精液后1周内的受精率。与一般报道的冻融家禽精液受精率相比，本实验的总体受精率（83%至93%）较高。第3天和第4天采集的精液受精率显著高于第1天或第2天采集的精液，表明在4天内频繁采集可提高精液的受精率。
