Coronado C, Vázquez M E, Cebolla A, Palomares A J
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain.
Plasmid. 1994 Nov;32(3):336-41. doi: 10.1006/plas.1994.1074.
A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.
本文描述了一种简单快速的测定质粒拷贝数的方法。真核荧光素酶基因用作标记，以标记几种知名载体的质粒衍生物，并通过测量光活性来确定质粒拷贝数。还描述了一种使用标准杂交程序通过光密度法估计质粒拷贝数的比较分析。
