Berezkina E V, Lipskaia L A, Grinchuk T M, Kovaleva Z V, Sorokina E A, Vasiukhin V I, Ignatova T N
Tsitologiia. 1994;36(7):687-95.
Stable mutant cells spEBR-5, resistant to ethidium bromide in concentration of 5 micrograms/ml, have been isolated by multistep selection in mouse myeloma cells sp2/0-Ag14. The spEBR-5 cells, selected with ethidium bromide, appeared to be cross resistant to unrelated drugs in connection with mdr/lb gene amplification and overexpression. Five specific chromosomal markers were detected in the karyotype of spEBR-5 cells as a result of chromosome structural rearrangement. No cytological manifestation of gene amplification such as HCR of chromosomes or DMs was found. A diffuse location of amplified sequences in chromosome(s) is suggested. The new mutant cell lines spEBR-5 can be used as a model for investigation of multidrug resistance mechanisms.
通过在小鼠骨髓瘤细胞sp2/0-Ag14中进行多步筛选，分离出了对浓度为5微克/毫升的溴化乙锭具有抗性的稳定突变细胞spEBR-5。用溴化乙锭筛选出的spEBR-5细胞似乎因mdr/lb基因扩增和过表达而对不相关药物产生交叉抗性。由于染色体结构重排，在spEBR-5细胞的核型中检测到五个特定的染色体标记。未发现基因扩增的细胞学表现，如染色体的均质染色区（HCR）或双微体（DMs）。提示扩增序列在染色体中呈弥散分布。新的突变细胞系spEBR-5可作为研究多药耐药机制的模型。
