[用于后续流式分选的蔗糖密度梯度染色体分级分离]

[Chromosome fractionation in a saccharose density gradient for subsequent flow sorting].

作者信息

Shatrova A N, Aksenov N D, Teslenko L V, Zenin V V

出版信息

Tsitologiia. 1994;36(7):708-12.

Abstract

The human chromosomes were obtained from lymphoblastoid cell line BOLD with normal karyotype, and separated on the basis of their size by velocity sedimentation at 190 g in sucrose gradient. Different chromosomal fractions were stained by applying fluorochromes with different DNA-base specificity: Hoechst 33258 and Olivomycine and were analysed using dual laser cell-sorter ATC-3000 (Brucker). Velocity sedimentation allowed to enrich certain fractions by individual chromosomes and thus speed up the sorting rate from 90 up to 200 chromosomes per second.

摘要

人类染色体取自具有正常核型的淋巴母细胞系BOLD，并通过在190 g下于蔗糖梯度中进行速率沉降，根据其大小进行分离。通过应用具有不同DNA碱基特异性的荧光染料（Hoechst 33258和橄榄霉素）对不同的染色体组分进行染色，并使用双激光细胞分选仪ATC - 3000（布鲁克公司）进行分析。速率沉降能够通过单个染色体富集某些组分，从而将分选速率从每秒90条染色体提高到200条染色体。