Kelsh R, Dawson I, Akam M
Department of Genetics and Wellcome/CRC Institute, Cambridge, UK.
Development. 1993 Jan;117(1):293-305. doi: 10.1242/dev.117.1.293.
We have cloned a homologue of the homeotic gene Abdominal-B from the locust Schistocerca gregaria and raised antibodies against the homeodomain and C-terminal flanking region of the encoded protein. This antibody stains the posterior abdomen of the developing Schistocerca embryo from 26% of development onwards, a stage when only the anterior of the abdomen is visibly segmented. By the time segmentation has reached the posterior of the abdomen, ABD-B protein is detectable only in the terminal segment (A11). Expression extends anteriorly as development proceeds to include the epidermis of all segments from A8p to A11, including the genital appendages of A9 and A10. The anal cerci, generally regarded as appendages of A11, remain unstained. This expression domain corresponds to the region within which the Abd-B r function of the Drosophila Abd-B gene is expressed. We detect no expression corresponding to the Abd-B m function in Drosophila.
我们从飞蝗（Schistocerca gregaria）中克隆了同源异型基因腹节B（Abdominal-B）的一个同源物，并制备了针对该编码蛋白同源结构域和C端侧翼区域的抗体。这种抗体从发育的26%起就对发育中的飞蝗胚胎后腹部进行染色，此时只有腹部前部可见分节。当分节到达腹部后部时，仅在末端节段（A11）可检测到ABD-B蛋白。随着发育进行，表达向前延伸，包括从A8p到A11所有节段的表皮，包括A9和A10的生殖附肢。通常被视为A11附肢的肛尾须仍未被染色。这个表达域对应于果蝇Abd-B基因的Abd-B r功能所表达的区域。我们未检测到与果蝇Abd-B m功能相对应的表达。
