Irvine K D, Botas J, Jha S, Mann R S, Hogness D S
Department of Biochemistry, Beckman Center, Stanford University School of Medicine, CA 94305.
Development. 1993 Jan;117(1):387-99. doi: 10.1242/dev.117.1.387.
The Drosophila homeotic gene Ultrabithorax (Ubx) encodes transcriptional regulatory proteins (UBX) that specify thoracic and abdominal segmental identities. Ubx autoregulation was examined by manipulating UBX levels, both genetically and with an inducible transgene, and monitoring the effect of these manipulations on the expression of Ubx and Ubx-lacZ reporter genes. Positive autoregulation by Ubx is restricted to the visceral mesoderm, while in other tissues Ubx negatively autoregulates. In some cases, negative autoregulation stabilizes UBX levels, while in others it modulates the spatial and temporal patterns of UBX expression. This modulation of UBX expression may enable Ubx to specify distinct identities in different segments. The upstream control region of Ubx contains multiple autoregulatory elements for both positive and negative autoregulation.
果蝇同源异型基因超双胸（Ubx）编码转录调节蛋白（UBX），该蛋白决定胸段和腹段的节段特性。通过遗传手段和诱导型转基因操作来调控UBX水平，并监测这些操作对Ubx和Ubx - lacZ报告基因表达的影响，以此研究Ubx的自我调节。Ubx的正向自我调节仅限于内脏中胚层，而在其他组织中Ubx进行负向自我调节。在某些情况下，负向自我调节可稳定UBX水平，而在其他情况下，它则调节UBX表达的时空模式。UBX表达的这种调节可能使Ubx在不同节段中确定不同的特性。Ubx的上游控制区包含用于正向和负向自我调节的多个自我调节元件。
